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Herbert Garrison (known as Janet Garrison between his two sex changes) is a recurring character in the South Park cartoon series. He is voiced by Trey Parker. For the first eight seasons of the series, the character was known as Mr. Garrison but in season 9 underwent sex reassignment surgery. The character was thereafter known to the other characters as Mrs. Garrison, despite being unmarried. In the season 12 episode, "Eek, a Penis!", he undergoes yet another sex change operation (back to a man). Garrison is particularly cynical, especially in comparison with the rest of South Park's adults, and he is one of the few characters to ever break the fourth wall on the show.

The Mr. Garrison character was in part inspired by a Kindergarten teacher Trey Parker had in the Metropolitan Nashville Public Schools who used a puppet named Mr. Hat as a teaching resource. Mr. Garrison was also inspired by a British Literature professor Parker had at the University of Colorado; Parker said the voice he uses for the character is a dead-accurate impression of him. Parker said he believes Mr. Garrison has become one of the most complex characters on South Park, particularly due to his ever-growing relationship with Mr. Hat and his sexuality and gender issues; Parker said of Mr. Garrison, "He's the soap opera element to the whole series. (He) has a real story going on."

Garrison also revealed racist tendencies in many episodes. In the episode "Chef Goes Nanners", Mr. Hat, his puppet, was a member of the local Ku Klux Klan, which Mr. Garrison resented and insisted to Chef that he wanted nothing to do with. However, in the later episode "Here Comes the Neighborhood", he tries to get rid of the "richers" (who were all black) using Klan techniques, such as wearing their robes or burning crosses. Although throughout the episode, both Mr. Garrison and the rest of the townsfolk make no reference to the "richers" being black (seemingly oblivious to the fact), in his last line of the episode that his intentions were at least in part racist ("at least we got rid of all those damn ni...[credits]). In the episode An Elephant Makes Love to a Pig he quotes: "Genetic engineering erases all of God's mistakes, like German people". In "Mr Hankey the Christmas Poo" and "It's Christmas in Canada", he suggests that the town get rid of all the Mexicans, but Mayor McDaniels repeatedly declines to do so. Exactly why he wishes to do this has not yet been explained. These are two examples of the many instances throughout the show's history where Mr Garrison has expressed contempt for the Mexicans, though in the episode "D-Yikes", Garrison relies on Mexicans to help him in a plan to thwart Persian Nightclub owners.

Despite the fact that he seems to portray racist manners, he sometimes will inform his student Eric Cartman of when the nine year old is doing something intolerant or offensive (as seen in "The Snuke").

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