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The Ambiguously Gay Duo is an American animated comedy sketch that debuted on The Dana Carvey Show before moving to its permanent home on Saturday Night Live. It is created and produced by Robert Smigel and J. J. Sedelmaier as part of the Saturday TV Funhouse series of sketches. It follows the adventures of Ace and Gary, voiced by Stephen Colbert and Steve Carell, two superhero whose sexual orientation is a matter of dispute, and a cavalcade of characters preoccupied with the question.

The Ambiguously Gay Duo is a parody of the stereotypical comic book superhero duo, clad in matching pastel turquoise tights, dark blue domino mask, and bright yellow coordinated gauntlets and boots. The shorts were intended to satirize suggestions that early Batman comics implied a homosexual relationship between the title character and his sidekick Robin, a charge most infamously leveled by Fredric Wertham in his 1954 book, Seduction of the Innocent.

The typical episode usually begins with the duo's arch-nemesis Bighead, a criminal mastermind with an abnormally large cranium. Bighead is usually briefing his henchmen on a plot for some grandiose plan for world domination, interrupted by a debate as to whether or not Ace and Gary (The Ambiguously Gay Duo) are gay. Once the crime is in process, the police commissioner calls on the superheroes to save the day, often engaging in similar debates with the chief of police.

Ace and Gary set out to foil the evil plan, but not before calling attention to themselves with outrageous antics and innuendo, and behaving in ways perceived by other characters as profligately homosexual, such as this example conversation from the first episode:

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