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Melinda Qiaolian May is a fictional character that originated in the Marvel Cinematic Universe before appearing in Marvel Comics. The character, created by Joss Whedon, Jed Whedon and Maurissa Tancharoen, first appeared in the 2013 pilot episode of Agents of S.H.I.E.L.D. and has continually been portrayed by Ming-Na Wen.

In season one, Melinda May is brought on to the recently resurrected S.H.I.E.L.D. agent Phil Coulson's team as pilot and field agent, to investigate superhumans and other related phenomena. She is shown to be in a relationship with fellow agent Grant Ward. Coulson tells agent Skye that May, during a mission in Bahrain in 2008, single-handedly took out a superhuman threat and several accomplices, saving a team of S.H.I.E.L.D. agents, though a young girl was killed in the crossfire. Left traumatized by the experience, May withdrew from field duty. Later, her trauma is shown to be gradually healing. She also ends her relationship with Ward. Unknown to Skye and Coulson, May is monitoring their conversation, and reporting to someone else. When the Hydra Uprising begins, May reveals that she knew the truth about Coulson's resurrection and was monitoring him on director Nick Fury's orders, and Coulson refuses to trust her any more. Feeling unwanted, she leaves, searching to find out the truth behind Coulson's resurrection. She finds the truth and brings it back to Coulson. May later incapacitates Ward, who was a double agent for Hydra, and after Ward's mentor John Garrett's death, goes with the team to the Playground to help restart S.H.I.E.L.D., under the newly promoted director Coulson.

In season two, May is Coulson's unofficial deputy. She later meets her ex-husband Andrew Garner. When another S.H.I.E.L.D. faction led by Robert Gonzales emerges and occupies their base, May helps Coulson to escape, but later accepts a position on Gonzales' board. It is revealed that in 2008 at Bahrain, the Inhuman Eva Belyakov and some gangsters took a S.H.I.E.L.D. team and several locals hostage, and May entered the building they were using to rescue the hostages. She found them acting out of character, and they attacked her, but she defeated them and killed Eva in a fight, before discovering Eva's daughter Katya was the real threat, having taken control of the hostages to feed on their pain. When Katya killed the Bahrainis she was controlling, May was forced to kill Katya. S.H.I.E.L.D. assumed Eva was the threat and Katya was caught in the crossfire, and May was lauded for saving the S.H.I.E.L.D. team single-handedly and given the nickname 'the Cavalry'. However she became deeply traumatized; her marriage to Garner also suffered, eventually leading to divorce. When the two S.H.I.E.L.D. factions unite, May becomes a member of Coulson's council of advisors. At the end of the season, May takes a break from S.H.I.E.L.D. to be with Garner.

In season three, May has been on leave from S.H.I.E.L.D. for six months and is looking after her father, who was injured in a car accident. Lance Hunter hopes that May will help him find and kill an absconding Ward, and notes that she suspects that Ward was behind her father's accident, but May is hiding from her life at S.H.I.E.L.D. and difficulties in her relationships with Garner and Coulson. May is eventually convinced by her father that getting back into her S.H.I.E.L.D. life by going with Hunter is the best thing for her, and the two leave to infiltrate the new ranks of Hydra. She later learns that Garner is the Inhuman serial killer Lash. May struggles with this revelation; she continues to work in S.H.I.E.L.D. as the agency attempts to bring down Gideon Malick and later Hive, losing Garner in this battle.

Melinda May has not been a contender in any CBUB matches.